CONSERVING OF THE RESOURCES
Evaluation of garden pea cultivars to salt stress tolerance
- 1 Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria
Abstract
Soil salinity is one of the main limiting factors for the plant growth and productivity. Response of three garden pea cultivars (Mira, Zornitsa and Ran 1) to salt stress in in vitro and in vivo conditions was studied. The in vitro stress was induced by adding of 0 (nontreated control), 50, 100 and 200 mM NaCI in culture medium. After two weeks the germination and seedling emergence rate were not significantly affected by 50 mM NaCl. In culture medium containing 200 mM NaCl only 13.3 – 43.3% of the seeds developed to the plants. The data indicated that increasing NaCl level the shoot and root lengths, and plant fresh weight were significantly reduced. In in vivo condition, such clear differences between the tested NaCl concentrations were not observed. Nevertheless, the applied salt stress more strongly affected the shoot fresh weight and root length than stem length. Among the three tested genotypes cultivar Zornitsa was the most tolerant one, proved in both in vitro and in vivo experiments.
Keywords
References
- Duzdemir, O., Kurunc, A., Unlukara, A. (2009). Response of pea (Pisum sativum) to salinity and irrigation water regime. Bulgarian Journal of Agricultural Science 15: 400-409.
- El Sayed, H., Sayed, A. El. (2011). Isolation and characterization of NaCl resistant calus line of field pea (Pisum sativum. L.) to salinity. Agriculture and Biology Journal of North America 2: 964-973.
- Flowers, T.J., Gaur, P.M., Gowda, C.L.L., Krishnamurthy, L., Samineni, S., Siddique, K.H.M. et al. (2009). Salt sensitivity in chickpea. Plant, Cell and Environ. 33: 490-509.
- Francois, L. E., Maas, E. V. (1994). Crop response to management on salt-affected soils. In: M. Pessarakli (Editor), Handbook of Plant and Crop Stress-Marcel Dekker Inc., New York, pp. 149-181.
- Gamborg, O. L., Miller, R. A., Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50(1): 151-158.
- Hernández, J. A., Jiménez, A., Mullineaux, P., Sevilla, F. (2000). Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant, Cell and Environment 23: 853-862.
- Kaya, M., Kaya, G., Kaya M. D., Atak, M. (2008). Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea (Cicer arietinum L.). Journal Zhejiang University Science B 9(5): 371-377.
- Maksimović, I., Putnik-Delić, M., Gani, I., Marić, J., Ili, Z., (2010). Growth, ion composition, and stomatal conductance of peas exposed to salinity. Central European Journal of Biology 5(5): 682-691.
- Materne, M., McNeil, D., Hobson, K., Ford R. (2007). Abiotic stresses. In: Yadav, S.S., McNeil, D. and Stevenson, P.C. (eds) Lentil: An Ancient Crop for Modern Times. Springer, Dordrecht, The Netherlands, 315-329.
- Miljuš-Djukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A., Maksimović, V. (2013). Differential response of three contrasting pea (Pisum arvense, P. sativum and P. fulvum) species to salt stress: assessment of variation in antioxidative defence and miRNA expression. Australian Journal of Crop Science 7(13):2145-2153.
- Murashige, t., Skoog, F. (1965). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497.
- Okcu, G., Kaya, M. D., Atak, M. (2005). Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agricultural and Forestry 29: 237-242.
- Pandolfi, C., Stefano, M., Shabala, S. (2012). Physiology of acclimation to salinity stress in pea (Pisum sativum). Environmental and Experimental Botany 84: 44-51.
- Piwowarczyk, B., Tokarz, K., Kamin´ska, I. (2016). Responses of grass pea seedlings to salinity stress in in vitro culture conditions. Plant Cell Tissue and Organ Culture 124(2): 227-240.
- Queiros, F., Fidalgo, F., Santos, I., Salema, R. (2007). In vitro selection of salt tolerant cell lines in Solanum tuberosum L. Biol. Plantarum, 51: 728-734.
- Rai, M., Kalia, R., Singh, R., Gangola, M., Dhawan, A. (2011). Developing stress tolerant plants through in vitro selection—An overview of the recent progress. Environmental and Experimental Botany 71: 89-98.
- Saxena, N. P., Johansen, C., Saxena M. C., Silim, S. N. (1993) Selection for drought and salinity tolerance in cool-season food legumes. In: Singh KB, Saxena MC (eds), Breeding for Tolerance in Cool Season Food Legumes. John Wiley & Sons, Ltd, Chichester, UK, pp. 245–270.
- Stanisavljevic, N., Savic, J., Jovanovic, Z., Miljus-Djukic, J., Radovic, S., Vinterhalter, D., Vinterhalter, B. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. Acta Physiological Plantarum 34: 1997-2006.
- Steppuhn, H., Volkmar, K. M., Miller, P. R. (2001). Comparing canola, field pea, dry bean, durum wheat crops grown in saline media. Crop Science 41: 1827-1833.
- Toker, C., Lluch, C., Tejera N.A., Siddique, K.H.M. (2007) Abiotic stresses. In: Yadav, S.S., Redden, R., Chen, W. and Sharma, B. (eds) Chickpea Breeding and Management. CAB International, Wallingford, UK, 474-496.
- Wang, W., Vinocur, B., Altman, A. (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1-14.