Mechanization in agriculture & Conserving of the resources
Vol. 68 (2024), Issue 3, pg(s) 112-115

CONSERVING OF THE RESOURCES

Influence of effective microorganisms on the biological activity of the soil in greenhouse pepper cultivation

  • 1 Agricultural academy, Maritsa Vegetable Crops Research Institute (MVCRI) – Plovdiv, Bulgaria

Abstract

The article presents the results of a study of the influence of effective microorganisms Trichoderma viride, Bacillus subtilis and Enterobacter cloaceae on the biological activity of the soil when growing pepper on the plot with mineral fertilization (fertilizer rate N22 P16 K20 kg/da). The experiment was carried out at the Maritsa Vegetable Crops Research Institute, Plovdiv in an unheated steel-glass greenhouse with the Pirouette F1 variety, planted according to the scheme (90/60/40 cm). The studied microbial substances were used as 0.1% aqueous solutions of the standardized biological preparations “Trichodermine” (Trichoderma viride, strain Trv1, titer 2.1010 c/ml); “Extrasol” (Bacillus subtilis, titer 1.1010 CFU/ml) and a bacterial preparation based on Enterobacter cloaceae (titer 1.1010 CFU/ml) at a consumption rate of 10 L of solution per 20 m2. The biological activity of the soil in the experiment was followed in different phenological phases of the development of the culture using the methodology for cultivating diluted aqueous soil solutions on solid nutrient media. As a result of the studies, it was established that the use of bioproducts based on Trihoderma viride, Bacillus subtilis and Enerobacter cloacae leads to optimization of the structural composition of the microbiome in the soil. Among the tested biopreparations with the greatest efficiency is the biopreparation “Trichodermine”. The introduction of the biopreparation “Trichodermine” increases the biogenicity of the soil by 12% towards the middle and by 74% – towards the end of the growing season. The agrochemical effectiveness of the application of the biopreparation “Trichodermine” to regulate the nitrogen-phosphate-potassium nutritional regime has been established. During the period of active harvesting, the content of mineral forms of nitrogen in the variant with “Trichodermine” increases to 50%, of mobile phosphorus – to 40%, of mobile potassium – to 71%. An article confirms the expediency and effectiveness of the application of biological preparations to optimize the structural composition of the microbiome in the soil and regulate the nitrogen-phosphate-potassium nutritional regime.

Keywords

References

  1. M. Abdelaziz, R. Pokluda, M. Abdelwahab, Cluj, Influence of compost, microorganisms and NPK fertilizer upon growth, chemical composition and essential oil production of Rosmarinus officinalis, Not Bot Hortic Agrobot 35, pp.1842–4309 (2007). Google Scholar
  2. S. Brahma, DB Phookan, Effect of nitrogen, phosphorus and potassium on yield and economics of broccoli [Brassica oleracea (L.) var. italica] cv. Pusa, Res Crops, 7, pp. 261–262 (2006)
  3. G. Mohammadi, A. Rostami Ajirloo, M. Ghobadi and A. Najaphy, Effects of non-chemical and chemical fertilizers on potato (Solanum tuberosum L.) yield and quality, J Med Plant Res, 7, pp. 36–42 (2013)
  4. T. Mao and X. Jiang, Changes in microbial community and enzyme activity in soil under continuous pepper cropping in response to Trichoderma hamatum MHT1134 application, Sci. Rep., 11, 21585 (2021) doi: 10.1038/s41598-021-00951-x. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  5. B. Anandaraj, A. Leema Rose Delapierre, Studies on influence of bioinoculants (Pseudomonas fluorescens, Rhizobium sp., Bacillus megaterium) in green gram, J Biosci Tech, 1, pp. 95–99 (2010)
  6. P.H. Graham, Ecology of the root-nodule bacteria of legumes. In: Dilworth MJ, James EK, Spent JI, Newton WE (eds) Nitrogen-fixing leguminous symbioses, Springer, Dordrecht, The Netherlands, pp 23–58 (2008)
  7. SL. Lim, S. Subramaniam, I. Zamzuri and G. Ami, Growth and biochemical profiling of artificiallyassociated micropropagated oil palm plantlets with Herbaspirillum seropedicae, J. Plant Interac, 13, pp. 173-181 (2018)
  8. S. Bobadi, P. Van Damme, Effect of nitrogen application on flowering and yield of eggplant (Solanum melongena L.), Commun Agric Appl Biol Sci, 68, pp. 5–13 (2003) PubMed Google Scholar
  9. Z . Guo, C. He, Y. Ma. H. Zhu, F. Liu, D. Wang, L. Sun, Effect of different fertilization on spring cabbage (Brassica oleracea L. var. capitata) production and fertilizer use efficiencies, Agric Sci, 2, pp. 208–212 (2011)
  10. B. Glick, Introduction to plant growth-promoting bacteria, In: Beneficial Plant-Bacterial Interactions, (2020)
  11. K. A. Hussein and J. H. Joo, Effects of several effective microorganisms (EM) on the growth of Chinese cabbage (Brassica rapa), Korean J. Soil Sci. Fert., 44(4), pp. 565- 574 (2011)
  12. A. Ibiene, J. Agogbua, I. Okonko and G. Nwachi, Plant growth promoting rhizobacteria (PGPR) as biofertilizer: Effect on growth of Lycopersicum esculentus, Journal of American Science, 8(2), pp. 318-324 (2012)
  13. T. Z. Kha, A. Kanarskii, Z. Kanarskaia, A. Shcherbakov, E. Shcherbakova, Prospects and Application of Paenibacillus in Industrial Biotechnology for Obtaining the Bio-Based Products for Agriculture, Vestnik of Volga State University of Technology. Ser.: Forest. Ecology. Nature Management, No 3 (47). pp. 74–84 (2020) https://doi.org/10.25686/2306-2827.2020.3.74
  14. A. Alfiky and L.Weisskopf, Deciphering Trichoderma– plant–pathogen interactions for better development of biocontrol application, J. Fungi, 7, 61 (2021) doi: 10.3390/jof7010061. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  15. U. Krauss, W. Soberanis, Biocontrol of Cocoa Pod Diseases with Mycoparasite Mixtures, Biological Control, v. 22 (2), pp. 149-158 (2001)
  16. K. Dou, J. Gao, C. Zhang, H. Yang, X. Jiang, J. Li, Y. Li, W. Wang, H. Xian, S. Li, Y. Liu, J. Hu and J. Chen, Trichoderma biodiversity in major ecological systems of China, J. Microbiol., 57, pp. 668–675 (2019) doi: 10.1007/s12275-019-8357-7. [PubMed] [CrossRef] [Google Scholar]
  17. K. C. Kumhar, K. Kumar, I. Arora, A. K. Bhatia, V. K. Batra and H. Raj, Management of Tomato Damping-off Disease in the Nursery Using of Trichoderma asperellum, International Journal of Economic Plants, v. 9 (2), 145–151 (2022)
  18. R. Tyśkiewicz, A. Nowak, E. Ozimek, J. Jaroszuk-ściseł, Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth, Int. J. Mol. Sci, v. 23(4), 2329 (2022) doi: 10.3390/ijms23042329. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  19. T. Borovikova, Methodical manual for soil microbiology, Krivoy Rog, p. 68 (2003) (Ru)
  20. D.G. Zvyagintsev, Methods of Soil Microbiology and Biochemistry, Moscow, p. 303 (1991) (Ru)
  21. N. M. Pidoplichko, Fungi-parasites of cultivated plants. The determinant, Kiev, Naukova Dumka, (1977)
  22. S. Murtazina, L. Gaffarova, M. Murtazin, A. Saimardanova, Evaluation of anthropogenic sustainability of agro-gray forest soil in intensive agriculture by change of its biological activity indicators, BIO Web of Conferences, 17, pp. 1–4 (2020)
  23. I. Druzhinina and C. Kubicek, Environmental and Microbial Relationships, The Mycota IV, 3rd edt., Berlin: Springer International Publishing, (2016) doi: 10.1007/978-3-319-29532-9

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