International Scientific Journals
of Scientific Technical Union of Mechanical Engineering "Industry 4.0"

In this study we investigate the kinetics of oxygen tension pО2) in skin tissue under the influence of the transcutaneous laser irradiation. The results of in vivo experimental measurements of pО2 by a method of transcutaneous oxygen monitoring (TcOM) are presented. The results show that under laser irradiation the value of tissue oxygenation increases and after approximately 10 minutes of exposure exceeds its initial level up to 1.6 times. The observed increase in pO2 indicates the process of photodissociation of oxyhemoglobin (HbO2) in skin blood vessels, which results in local O2 increase in the tissue. Such laser-induced enrichment of tissue oxygenation can be used in phototherapy of pathologies, where the elimination of local tissue hypoxia is critical.

In the present work we have investigated the parameters of peripheral blood flow by using a non-invasive method for processing photoplethysmograms (PPGs). The developed original technique allows us to register a photoplethysmogram, isolate the pulse wave and determine the duration of cardio intervals, the duration of the anacrotic and dicrotic phases, the duration of fast and slow blood filling, the expulsion period, the dicrotic and diastolic indices, temporal and amplitude parameters of vascular tone, etc. We observe three types of PPGs after taking medicaments Corvitol-50 and nitroglycerin. The analysis of the amplitude-temporal characteristics of PPGs allows the determination of key diagnostic parameters.

Immobilization of proteins on a surface plasmon resonance (SPR) transducer is a delicate procedure since loss of protein bioactivity can occur upon contact with the untreated metal surface. Solution to the problem is the use of an immobilization matrix having a complex structure. In this paper we study the impact of direct immobilization of heme proteins (hemoglobin (Hb) and myoglobin (Mb)) on their bioactivity. We have used spin coating, for direct immobilization and matrix-assisted pulsed-laser evaporation (MAPLE) [1] for elaboration of the SPR biochip. The performance of both SPR chips – direct and MAPLE immobilized, was studied by SPR registration of the binding activity of Hb and Mb ligands with carbon monoxide (CO), carbon dioxide (CO2) and nitride oxide (NO). The experimental facts showed that direct immobilization of an intact molecule was achieved