Silk-based scaffolds are specifically investigated in various tissue engineering applications, including for cartilage, bone, nerve, muscle, skin, or corneal regeneration. Guiding muscle cell growth is a challenging task in muscle tissue engineering. In this work, a selfassembled silk fibroin thin films were processed by ultra-short laser radiation to investigate its potential for guiding muscle cell proliferation. The ultra-short laser processing of silk fibroin (SF) have produced micro channels which are suitable for self-assembling and orientation of muscle cells and provides a niche for its attachment. Silk fibroin is an excellent candidate as a biomaterial for tissue engineering applications. Moreover, bacterial biofilm formation on surfaces are associated with persistent microbial contamination. Thus, recently new approaches are needed to impede bacterial surface colonization. Using femtosecond laser irradiation (wavelength 800 nm), laser-induced surface microstucturing is applied to achieve non-thermal, precise, and crack free surface processing with different topographical designs on silk fibroin thin films in order to enhance repelling of bacteria attachment.
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