Review of the chemical and active compounds of the lyophilized essential oli of Thymus vulgaris L. with special reference ot the preservation of the quality of the finished product

  • 1 Faculty of Medicine, University of Montenegro, Krusevac, Podgorica, Montenegro


The article reviews the chemical and active compounds of the lyophilized essential oil of Thymus vulgaris L. and its preservation qualities. Thymus vulgaris is a medicinal plant belonging to the Lamiaceae family, with a long history of use for its therapeutic properties. The essential oil of T. vulgaris has been widely studied for its antimicrobial, antioxidant, and anti-inflammatory activities. The amounts and types of chemical compounds can vary depending on the genetic differences or environmental conditions of the plant materials. The primary components of the essential oil responsible for most of its biological effects include thymol, carvacrol, and p-cymene. The essential oil of T. vulgaris has been reported to exhibit a wide range of pharmacological activities, including antimicrobial, antioxidant, anti-inflammatory, antifungal, and antiviral activities. In recent years, there has been increasing interest in the use of T. vulgaris essential oil as a natural preservative in food products.



  1. Pedersen, J. A. (2000). Distribution and taxonomic implications of some phenolics in the family Lamiaceae determined by ESR spectroscopy. Biochemical systematics and Ecology, 28(3), 229-253.
  2. Ghasemi Pirbalouti, A., Emami Bistghani, Z., & Malekpoor, F. (2015). An overview on genus Thymus. Journal of Medicinal Herbs,, 6(2), 93-100.
  3. Stahl-Biskup E, Venskutonis R. 2012. Thyme. Handbook of herbs and spices, Elsevier: 499-525.
  4. Stahl-Biskup, E., & Saez, F. (2002, September 5). Thyme. CRC Press.
  5. Thompson, J. D., Chalchat, J. C., Michet, A., Linhart, Y. B., & Ehlers, B. (2003). Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes. Journal of chemical ecology, 29, 859-880.
  6. Thompson, J. D., Manicacci, D., & Tarayre, M. (1998). Thirty-Five Years of Thyme: A Tale of Two Polymorphisms. BioScience, 48(10), 805–815.
  7. Dajic-Stevanovic, Z., Sostaric, I., Marin, P., Stojanovic, D., & Ristic, M. (2008). Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition. Archives of Biological Sciences, 60(3), 475–483.
  8. Basch, E., Ulbricht, C., Hammerness, P., Bevins, A., & Sollars, D. (2004, January). Thyme (Thymus vulgarisL.), Thymol. Journal of Herbal Pharmacotherapy, 4(1), 49–67.
  9. Borugă, O., Jianu, C., Mişcă, C., Goleţ, I., Gruia, A. T., & Horhat, F. G. (2014). Thymus vulgaris essential oil: chemical composition and antimicrobial activity. Journal of medicine and life, 7 Spec No. 3(Spec Iss 3), 56–60.
  10. Golparvar, & Hadipanah. (2023). A review of the chemical composition of essential oils of thymus species in Iran. Research on Crop Ecophysiology, 18/1(Issue 1), 25–51.
  11. Rota, M. C., Herrera, A., Martínez, R. M., Sotomayor, J. A., & Jordán, M. J. (2008, July). Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control, 19(7), 681–687.
  12. European Pharmacopoeia, 10th Edition 2019, English. (2019, January 1).
  13. Aeschbach, R., Löliger, J., Scott, B., Murcia, A., Butler, J., Halliwell, B., & Aruoma, O. (1994, January). Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food and Chemical Toxicology, 32(1), 31–36.
  14. Grosso, C., Figueiredo, A. C., Burillo, J., Mainar, A. M., Urieta, J. S., Barroso, J. G., Coelho, J. A., & Palavra, A. M. F. (2010, July). Composition and antioxidant activity of Thymus vulgaris volatiles: Comparison between supercritical fluid extraction and hydrodistillation. Journal of Separation Science, 33(14), 2211–2218.
  15. Khaksar, V., Krimpen, M. V., Hashemipour, H., & Pilevar, M. (2012). Effects of Thyme Essential Oil on Performance, Some Blood Parameters and Ileal Microflora of Japanese Quail. The Journal of Poultry Science, 49(2), 106–110.
  16. Posgay, M., Greff, B., Kapcsándi, V., & Lakatos, E. (2022, October). Effect of Thymus vulgaris L. essential oil and thymol on the microbiological properties of meat and meat products: A review. Heliyon, 8(10), e10812.
  17. Gaidhani, K. A., Harwalkar, M., Bhambere, D., & Nirgude, P. S. (2015). Lyophilization/freeze drying–a review. World journal of pharmaceutical research, 4(8), 516-543.
  18. Nireesha, G. R., Divya, L., Sowmya, C., Venkateshan, N. N. B. M., & Lavakumar, V. (2013). Lyophilization/freeze drying-an review. International journal of novel trends in pharmaceutical sciences, 3(4), 87-98.
  19. Ratti, C. (2001, September). Hot air and freeze-drying of high-value foods: a review. Journal of Food Engineering, 49(4), 311–319.
  20. Thamkaew, G., Sjöholm, I., & Galindo, F. G. (2020, May 19). A review of drying methods for improving the quality of dried herbs. Critical Reviews in Food Science and Nutrition, 61(11), 1763–1786.
  21. Antal, Tamás & Chong, Chien Hwa & Law, Chung & Sikolya, László. (2014). Effects of freeze drying on retention of essential oils, changes in glandular trichomes of lemon balm leaves. International Food Research Journal. 21. 387-394.
  22. Venskutonis, P. (1997, June). Effect of drying on the volatile constituents of thyme (Thymus vulgaris L.) and sage (Salvia officinalis L.). Food Chemistry, 59(2), 219–227.
  23. Venskutonis, R., Poll, L., & Larsen, M. (1996, March). Influence of Drying and Irradiation on the Composition of Volatile Compounds of Thyme (Thymus vulgaris L.). Flavour and Fragrance Journal, 11(2), 123–128.<123::aid-ffj555>;2-1
  24. Sárosi, S., Sipos, L., Kókai, Z., Pluhár, Z., Szilvássy, B., & Novák, I. (2013, April). Effect of different drying techniques on the aroma profile of Thymus vulgaris analyzed by GC–MS and sensory profile methods. Industrial Crops and Products, 46, 210– 216.
  25. Hazarika, U., & Gosztola, B. (2020, December 30). Lyophilization and its effects on the essential oil content and composition of herbs and spices – A review. Acta Scientiarum Polonorum Technologia Alimentaria, 19(4), 467–473.
  26. Grigore, A., Paraschiv, I. N. A., Colceru-Mihul, S., Bubueanu, C., Draghici, E., & Ichim, M. (2010). Chemical composition and antioxidant activity of Thymus vulgaris L. volatile oil obtained by two different methods. Romanian Biotechnological Letters, 15(4), 5436-5443.
  27. Imelouane, B., Amhamdi, H., Wathelet, J. P., Ankit, M., Khedid, K., & El Bachiri, A. (2009). Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from Eastern Morocco. Int. J. Agric. Biol, 11(2), 205-208.
  28. Ballester-Costa, C., Sendra, E., Fernández-López, J., Pérez-Álvarez, J. A., & Viuda-Martos, M. (2013). Chemical composition and in vitro antibacterial properties of essential oils of four Thymus species from organic growth. Industrial Crops and Products, 50, 304-311.
  29. De Lisi, A., Tedone, L., Montesano, V., Sarli, G., & Negro, D. (2011). Chemical characterisation of Thymus populations belonging from Southern Italy. Food Chemistry, 125(4), 1284-1286.
  30. Kowalski, R., & Wawrzykowski, J. (2008, December 3). Essential oils analysis in dried materials and granulates obtained from Thymus vulgaris L., Salvia officinalis L., Mentha piperita L. and Chamomilla recutita L. Flavour and Fragrance Journal, 24(1), 31–35.
  31. Betts, T. J. (2001, November). Chemical characterisation of the different types of volatile oil constituents by various solute retention ratios with the use of conventional and novel commercial gas chromatographic stationary phases. Journal of Chromatography A, 936(1–2), 33–46. 5
  32. Pichersky, E., Noel, J. P., & Dudareva, N. (2006, February 10). Biosynthesis of Plant Volatiles: Nature’s Diversity and Ingenuity. Science, 311(5762), 808–811.
  33. Omidbeygi, M., Barzegar, M., Hamidi, Z., & Naghdibadi, H. (2007, December). Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control, 18(12), 1518–1523.
  34. Sefidkon, F., & Jamzad, Z. (2005, June). Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chemistry, 91(1), 1– 4.
  35. Viuda-Martos, M., Mohamady, M., Fernández-López, J., Abd ElRazik, K., Omer, E., Pérez-Alvarez, J., & Sendra, E. (2011, November). In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control, 22(11), 1715–1722.
  36. Tomaino, A., Cimino, F., Zimbalatti, V., Venuti, V., Sulfaro, V., De Pasquale, A., & Saija, A. (2005, March). Influence of heating on antioxidant activity and the chemical composition of some spice essential oils. Food Chemistry, 89(4), 549–554.
  37. Viuda-Martos, M., El Gendy, A. E. N. G. S., Sendra, E., Fernández-López, J., Abd El Razik, K. A., Omer, E. A., & Pérez- Alvarez, J. A. (2010, July 28). Chemical Composition and Antioxidant and Anti-Listeria Activities of Essential Oils Obtained from Some Egyptian Plants. Journal of Agricultural and Food Chemistry, 58(16), 9063–9070.
  38. Satyal, P., Murray, B., McFeeters, R., & Setzer, W. (2016, October 27). Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations. Foods, 5(4), 70.
  39. Soković, M., Glamočlija, J., Ćirić, A., Kataranovski, D., Marin, P. D., Vukojević, J., & Brkić, D. (2008, January). Antifungal Activity of the Essential oil ofThymus vulgarisL. and Thymol on Experimentally Induced Dermatomycoses. Drug Development and Industrial Pharmacy, 34(12), 1388–1393.
  40. Fani, M., & Kohanteb, J. (2017, April 11). In Vitro Antimicrobial Activity of Thymus vulgaris Essential Oil Against Major Oral Pathogens. Journal of Evidence-Based Complementary & Alternative Medicine, 22(4), 660–666.
  41. Inouye, S., Takizawa, T., & Yamaguchi, H. (2001, May 1). Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. Journal of Antimicrobial Chemotherapy, 47(5), 565–573.
  42. Nikolić, M., Glamočlija, J., Ferreira, I. C., Calhelha, R. C., Fernandes, N., Marković, T., Marković, D., Giweli, A., & Soković, M. (2014, January). Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Industrial Crops and Products, 52, 183–190.
  43. Blanc, A. R., Sortino, M. A., Butassi, E., & Svetaz, L. A. (2023, November). Synergistic effects of Thymus vulgaris essential oil in combination with antifungal agents and inhibition of virulence factors of Candida albicans. Phytomedicine Plus, 3(4), 100481.
  44. Giordani, R., Regli, P., Kaloustian, J., Mikaïl, C., Abou, L., & Portugal, H. (2004, December). Antifungal effect of various essential oils against Candidaalbicans. Potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris. Phytotherapy Research, 18(12), 990–995.
  45. Sárosi, S., Sipos, L., Kókai, Z., Pluhár, Z., Szilvássy, B., & Novák, I. (2013, April). Effect of different drying techniques on the aroma profile of Thymus vulgaris analyzed by GC–MS and sensory profile methods. Industrial Crops and Products, 46, 210– 216.
  46. Rocha R. P. (2011, December 31). Influence of drying process on the quality of medicinal plants: A review. Journal of Medicinal Plants Research, 5(33).

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