Development of Software Application in the Evaluation of Erythrocyte Aggregation Studied with Microfluidic Device

  • 1 Dept. of Mechatronics, Institute of Mechanics, Bulgarian Academy of Sciences, Sofia, Bulgaria; Center of Competence at Mechatronics and Clean Technologies – MIRACle, Sofia, Bulgaria
  • 2 Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria; University Obstetrics and Gynecology Hospital “Maichin Dom”, Sofia, Bulgaria
  • 3 Center of Competence at Mechatronics and Clean Technologies – MIRACle, Sofia, Bulgaria; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria


The main focus of this research is on the elaboration of the combined experimental approach and image analysis (based on the specialized software environments) to the research of erythrocyte aggregation, which is evaluated by the microfluidic device BioFlux. To realize a precise evaluation of the erythrocyte aggregation index based on the obtained, during the experiment images, are applied image processing toolbox from ImageJ and an elaborated computer program in IntelliJ IDEA (integrated development environment) as well. The obtained results for the index of erythrocyte aggregation, based on the developed methodology, show a statistically significant difference between the two studied groups with preeclampsia and healthy pregnant women.



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