Recycled waste paper is a raw material for the paper industry. One of the most important processes in the recycling of waste paper, for the ability to print, is deinking. One perspective and ecological method for implementing that is the flotation process with enzymatic treatment. The aim of this study is to investigate the deinking of secondary fibrous material, obtained from waste paper, by flotation of offset printed paper by the use of different types of enzymes – lipase, cellulase, amylase, xylanase, on the process of deinking by flotation. In this research are being investigated various proportions of enzymes, and combinations thereof with surfactants. Their ability of deinking is calculated. As a result the treatment of the secondary fibers with the enzymes, improves the papermaking properties. The improved recovery papermaking properties of secondary fibers by the effect of enzymes, is able to increase the number of cycles of use of waste paper and to improve some parameters of the paper produced from secondary fiber. The optical properties of the obtained deinked fiber material are investigated and the possibilities for its usage in the composition of various types of paper and paperboard.