An efficient combination of water treatment and electricity generation by different microorganisms

  • 1 University of Mining and Geology “St. Ivan Rilski”
  • 2 Sofia University “St. Kliment Ohridski”


A sample of acid drainage waters generated after rainfall in a dump of low-grade copper ores and mining wastes was tested under laboratory conditions to combine the treatment of such waters with the subsequent generation of electricity. This combination was possible and efficient due to the fact that the same microorganisms participated in both processes, i.e. in the water treatment and in the electricity generation. These microorganisms were heterotrophs related to two main groups: of sulphate-reducing and iron-reducing bacteria. These bacteria were anaerobes, with efficient growth and activity at pH within 3.0 – 3.5 using the organic donors of energy and avoiding the precipitation of the trivalent iron



  1. Du, Z., Li, H., Gu, T., 2007. A state of the art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy, Biotechnology Advances, 25, 464–482.
  2. Escobar, B., Bustos K., Morales, G. and Salazar, O., 2008. Rapid and specific detection of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans by PCR, Hydrometallurgy, 92, 102–106.
  3. Groudev, S. N., 2015. Biohydrometallurgy in Bulgaria – achievements and perspectives, Acta Microbiologica, 1, 81 – 92.
  4. Karavaiko, G.I., Rossi, G., Agate, A.D., Groudev, S.N. and Avakyan, Z.A. , 1988. Biogeotechnology of Metals. Mannual GKNT Center for International Projects, Moscow.
  5. Sanz, J.L. and Köchling, T., 2007. Molecular biology techniques used in wastewater treatment: An overview. Process Biochemistry 42, 119–133.
  6. Spasova, I., Nicolova, M., Georgiev, P., Groudev, S., 2014. Mine waters cleaning connected with electricity generation, Annual of the University of Mining and Geology, Sofia, 57 (II), 137–139.
  7. Spasova, I., Nicolova, M., Georgiev, P., Groudev, S., 2016. Bioleaching of metals from a waste ore in connection with copper recovery, environment protection and electricity generation, Annual of the University of Mining and Geology, Sofia, 59 (II), 167–171.
  8. Rabaey, K., Verstraete, W., 2005. Microbial fuell cells: novel biotechnology for energy generation, Trends in Biotechnology, 23 (6), 291–298.

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