MATHEMATICAL MODELLING OF MEDICAL-BIOLOGICAL PROCESSES AND SYSTEMS

Modeling and Analysis of Image Data of Blood Clots Formed at Different Fibrinogen Concentration in Patients with Type 2 Diabetes Mellitus

  • 1 Institute of Mechanics, Bulgarian Academy of Sciences, Sofia, Bulgaria; Center of Competence at Mechatronics and Clean Technologies – MIRACle, Sofia, Bulgaria
  • 2 Institute of Physical Chemistry “Rostislaw Kaischew”, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • 3 Center of Competence at Mechatronics and Clean Technologies – MIRACle, Sofia, Bulgaria; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria

Abstract

The present study aims to model and analyze the images of induced in vitro blood clots from healthy donors and patients with type 2 diabetes mellitus (T2DM). By using the BioFlux microfluidic system, blood clots are induced at varying shear rates, and for different fibrinogen concentrations: native and highly modified. The fiber diameter of blood clots is analyzed by the scanning electron microscope (SEM). The obtained images of blood clots are imported as input data into the Image J software environment, after which obtained results for the area, number, and fibrin fiber diameter of blood clots, are further processed in a program developed in IntelliJ IDEA. It is found that patients with T2DM at the native concentration of fibrinogen at all studied shear rates form more blood clots having a larger total area, in comparison with the control group. At the higher modified fibrinogen concentration with increasing shear rate, the group with T2DM forms a smaller number of blood clots with a larger area, compared to healthy donors. From the SEM images, it is found, that denser fibrin networks are formed with increased fibrinogen concentration, which contains numerous thick fibrin fibеrs in healthy and diabetic individuals.

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